5A). Copyright © 2020 by The American Society of Plant Biologists.
The BY2 cell culture is a heterotrophic system because of the lack of photosynthetic activity, and the carbon source in the medium is Suc (88 mm). In contrast, GUS messengers were detected in roots for all the promoters studied. It is likely that in the p0.8VvHT1-GUS construct, the effect of two sugar activation elements is antagonized by the presence of two AMYBOX2 sugar repression motifs. Part of complex regulatory web. Q: A: What is GUS abbreviation? Two opposite types of organs, adult leaves (source), and roots (sink), were chosen to study the steady-state level of GUS transcripts. This provided evidence that the non-cleavable disaccharides used in this study were also non-metabolizable (Fig. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. RNA gel-blot analysis of GUStranscripts accumulation under Glc induction of VvHT1promoter activity in tobacco BY2 transgenic cell suspension and mRNA quantification by using a Bio-Imaging Analyzer. 5B, C and D). Moreover, in both cultures, the onset of the stationary phase was correlated with Glc depletion in the medium. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. à lagent de sélection, 2/ LADN-T peut avoir
They describe the first example of induction of a putative monosaccharide transporter from higher plants, induced by its own substrate (i.e. Because palatinose is not digested, this indicates that the Suc effect cannot be simply because of Glc generated by an extracellular invertase. Aussi, l'existence d'activité béta-galactosidase Surprisingly, there was no significant difference in the level of expression among the three promoter lengths studied. Conversely, the disaccharide lactulose, which lacks a glucosyl component, did not affect VvHT1 promoter expression. 5B), whereas Fru did not induce any rise in GUS activity. The positions of putative sugar-responsive elements are shown with respect to the translation initiation start. Marrakech, 40000, Morocco, Email: baaziz@uca.ac.ma The expression also differs in sensitivity to environmental factors, such as pathogen attack or sugar concentration. Recently, a DNA-binding protein inducing the expression of VvHT1 was identified by using the proximal 160-bp part of VvHT1 promoter, encompassing the two positive sugar response motifs, as a bait in the one-hybrid approach (B. Cakir, A. Agasse, S. Delrot, and R. Atanassova, unpublished data). Induction of VvHT1 expression by Suc and palatinose in grape suspension cells (Fig. GUS relative activity in suspension cells carryingp0.3VvHT1/GUS, p0.8VvHT1/GUS,p2.4VvHT1/GUS, or p35S/GUS in response to the addition of different sugars.
nest pas transformée, mais naturellement résistante
Travaux pratiques Master Biotechnologies et Amélioration des From a functional point of view, these transporters may vary in substrate specificity and in their affinity for monosaccharides. endogène chez bon nombre de plantes fait que cette méthode Palatinose also induced an increase in VvHT1 expression.
For each construct, the control is the same transformed cell suspension without treatment. Plants expressing GUS are normal, healthy and fertile. The data dealing with sugar control of gene expression of monosaccharide transporters, and also Suc transporters, are scarce and sometimes contradictory. All GUS measurements presented were confirmed in three independently transformed suspensions and obtained on a total of six to 12 measurements coming from two to four independent experiments. SUT2, a putative sucrose sensor in sieve elements.
Marrakech
(gènes marqueurs), Promoteurs et régulation Position over or under the promoter line stands for plus or minus strand, respectively. Phone: 212524434649 (post 513) résistance intrinsèque, peuvent résister à When the p35S-GUS transgenic suspension was treated with exogenous sugars, there was no detectable change of GUS activity (Fig.5A). Successive deletions of the distal part of the promoter (p2.4VvHT1, p0.8 VvHT1, and p0.3VvHT1), with specific sites for restriction enzymes and primers used for chimeric pVvHT1/GUSfusions, are shown.
Depuis Moreover, the effect of Glc was stronger with 0.3VvHT1 promoter than with 0.8VvHT1 one. Looking for online definition of GUS or what GUS stands for?